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LI-COR/IRDye®680LT链霉亲和素/[{大小：0.5 mg，价格类型：默认，价格美元：135.0，价格英镑：152.0，详细说明：IRDye 680LT链霉亲和素作为缓冲液提供，缓冲液中含有10 mM磷酸盐、183 mM NaCl和2.7 mM KCl，pH 7
IRDye 680LT链霉亲和素作为缓冲液提供，缓冲液中含有10 mM磷酸盐、183 mM NaCl和2.7 mM KCl，pH 7
IRDye 680LT Streptavidin is supplied as a liquid in buffer containing 10 mM phosphate, 183 mM NaCl, 2.7 nM KCl, pH 7.4 with sodium azide 0.005% (w/v) as a preservative.
To use, centrifuge briefly before use to eliminate aggregates that may have formed in solution. This will reduce non-specific background staining. A final concentration of 0.2 to 1.0 µg/ml (1:1,000 to 1:5,000) is usually satisfactory for most applications; however, appropriate dilution may need to be determined empirically.
For membrane-based applications and In-Gel Westerns, it is recommended to add SDS (0.02% to 0.1% final concentration), in addition to Tween® 20 (0.1 to 0.2% final concentration) during the detection incubation step to reduce non-specific background staining.
Recommended Dilutions
| Application | Suggested Range | Tween 20* | SDS* |
|---|---|---|---|
| Odyssey® Western blot detection | 1:1,000 - 1:5,000 | 0.1 - 0.2% (v/v) | 0.02 - 0.1% (v/v) |
| Other | User optimized | User optimized | User optimized |
Optimum dilutions will vary and should be determined empirically.
* Added to reduce non-specific background staining.
See the complete line of IRDye Streptavidins.
