IRDye 800CW PEG Contrast Agent (25-60 kDa) is a non-specific imaging agent intended to exploit enhanced permeability and retention (EPR) in tumor biology.

EPR is a common characteristic of tumor vasculature. The vascular endothelium in the tumor microenvironment is often discontinuous, allowing molecules to diffuse into the surrounding tumor tissue.^{1, 2} In addition to EPR, the lymphatic drainage for these regions is poor.³ Hence, larger molecules tend to accumulate.

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Applications

• Vascular/Lymphatic Imaging
• Molecular Activity Measurements

Absorbance and Emission Spectra

Data Examples

In appropriate mouse models, the agent highlights surface vasculature for approximately 0.5 hour post-injection as seen in Figure 1. Approximately 4 hours post-injection, retention of the labeled macromolecule is visible in the tumor (Figure 2). At 9 hours post-intravenous injection the tumor is well defined (Figure 3).

IRDye 800CW PEG Contrast Agent may also be used effectively as a lymph tracking agent when given intradermally (Figure 4). Images captured on the Pearl^® Imaging System.

References

1. Vasey, P.A., et al. (1999) Clin, Cancer Res, 5:83-94.
2. Matsumura, Y. and H. Maeda. (1986) Cancer Res, 46:6387-6392.
3. Seymour, L.W. (1992) Crit. Rev. Ther. Drug Carrier Syst, 9(2):135-187.